A team of researchers from the University of Illinois got the highest recorded levels of putting genes into human cells with the CRISPR-Cas9 gene-transformed system. Such a thing is a needed phase for controlling CRISPR for clinical gene-treatment purposes.
So, by chemically twisting the ends of the DNA to be added, the new method is up to five times more effective than current procedures. Researchers noticed enhancements at different genetic places tested in a human kidney cell line. They saw a 65 % insertion at one site where the last high level had reached 15 %.
Conducted by chemical and biomolecular engineering professor Huimin Zhao, the team succeeded in finding vital information. Moreover, researchers have discovered CRISPR to be an essential item to turn off, or “knock out,” a gene. However, present in our cells, it has not been an advantageous way to insert or “knock-in” a gene.
CRISPR Twisting DNA Fragments Display the Highest Efficiency Level Before Putting Yields
Professor Zhao stated: “A good knock-in method is important for both gene-therapy applications and for basic biological research to study gene function.” Looking for a plan to enhance efficiency, Zhao’s team searched at 13 different ways to transform the added DNA.
He later detailed: “With a knock-in method, we can add a label to any gene, study its function and see how gene expression is affected by cancer or changes in chromosome structure. Or for gene-therapy applications, if someone has a disease caused by a missing gene, we want to be able to insert it.”
Researchers also tested inserting end-transformed DNA parts of different dimensions at many points in the genome, utilizing CRISPR-Cas9 to target specific places for insertion accurately. They discovered efficiency enhanced two to five levels, even when putting bigger DNA fragments – the most challenging addition to realize.